Expression of RSV-specific proteins in RSV-transformed mammalian cells

Expression of RSV-specific proteins was analysed in two virogenic and three helper-dependent RSV-transformed mammalian cell lines by the radioimmunoprecipitation technique. In all cell lines the only product of the gag gene was represented by the precursor Pr76gag without its further processing. The env gene was expressed in several lines in the form of 85K protein, but the product of this gene was absent in TWERC cells. The src gene product (pp60src) was identified by the protein kinase assay in all cells. In the kinase reaction in vitro, this protein can also phosphorylate some cellular proteins (130K, 34-36K and others) and the heavy chain of IgG.