Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature influences on different human alpha interferon activities.

N Hirai, N O Hill, K Osther

    Journal of Interferon Research
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Synthesis, Biological Activity, and Metabolism of 8',8',8'-Trideuteroabscisic Acid.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Synthesis and Biological Activities of 8'-Methylene- and 8'-Methylidyneabscisic Acids.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Phenylphenalenone-type Phytoalexins from Unripe Buñgulan Banana Fruit.

    Bioscience, biotechnology, and biochemistry·2016
    Same author

    Atmospheric Humidity Influences Oviposition Rate of Tetranychus urticae (Acari: Tetranychidae) Through Morphological Responses of Host Cucumis sativus Leaves.

    Journal of economic entomology·2015
    Same author

    Population genetic structure and Wolbachia infection in an endangered butterfly, Zizina emelina (Lepidoptera, Lycaenidae), in Japan.

    Bulletin of entomological research·2014
    Same author

    Prophylactic intra-arterial administration of fasudil hydrochloride for vasospasm following subarachnoid haemorrhage.

    Acta neurochirurgica. Supplement·2014
    Same journal

    Rapid activation of the interferon-gamma signal transduction pathway by inhibitors of tyrosine phosphatases.

    Journal of interferon research·1994
    Same journal

    Interferon-stimulated response element and NF kappa B sites cooperate to regulate double-stranded RNA-induced transcription of the IP-10 gene.

    Journal of interferon research·1994
    Same journal

    Interferon-alpha-induced biologic modifications in patients with chronic myelogenous leukemia.

    Journal of interferon research·1994
    Same journal

    Modulation of peripheral leukocyte counts and bone marrow function in mice by oral administration of interleukin-2.

    Journal of interferon research·1994
    Same journal

    IDO mutants cross resistant to type I interferon retain p91-dependent gene induction.

    Journal of interferon research·1994
    Same journal

    Interferon-alpha 2 produced by normal human leukocytes is predominantly interferon-alpha 2b.

    Journal of interferon research·1994
    See all related articles

    Incubation temperature influences human leukocyte-derived alpha interferon (HuIFN-alpha) activity. Elevated temperatures enhance antiviral and antiproliferative effects but do not impact natural killer (NK) cell augmentation.

    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Human leukocyte-derived alpha interferon (HuIFN-alpha) is crucial for immune responses.
    • Understanding how physiological temperature variations affect HuIFN-alpha's biological activities is important for its therapeutic applications.

    Purpose of the Study:

    • To investigate the in vitro effects of different physiological incubation temperatures on the antiviral, antiproliferative, and natural killer (NK) cell augmenting activities of HuIFN-alpha.

    Main Methods:

    • Antiviral activity assessed using plaque-reduction assay and cytopathogenic effect (CPE) inhibition.
    • Antiproliferative activity evaluated on various cell lines (Daudi, G-361 melanoma, PLC/PRF/5 hepatoma).
    • NK cell activity augmentation measured in peripheral blood lymphocytes (PBL) after incubation with HuIFN-alpha at different temperatures.

    Related Experiment Videos

    Main Results:

    • Antiviral activity showed varied responses, generally higher at 38-39°C compared to 37°C.
    • Antiproliferative activity was enhanced at elevated temperatures for most cell lines, with some exceptions.
    • NK cell activity was reduced at 41°C when cells were incubated without IFN, but IFN's augmenting effect on NK activity was consistent across tested temperatures.

    Conclusions:

    • Elevated incubation temperatures can potentiate the antiviral and antiproliferative activities of HuIFN-alpha.
    • The temperature-dependent effects on HuIFN-alpha activity are specific to the biological function being measured.
    • HuIFN-alpha's capacity to augment NK cell activity remains largely unaffected by physiological temperature variations.