Effect of queuine on protein biosynthesis in mammalian cells

Administration of queuine into intact cells has an affected protein biosynthesis in vivo. In C3H2K mouse kidney cells, synthesis of a protein having a molecular weight of 45,000 was prevented by addition of queuine into the culture medium. In the case of SA-1 cells, which are variant cells of mouse mammary tumor cells, a protein whose molecular weight was 95,000 and located in the cytosol of its cells was changed to a more basic protein by administration of queuine.