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Related Experiment Videos

Angiotensin II receptors in chromatin.

R N Re, D L Vizard, J Brown

    Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension
    |December 1, 1984
    PubMed
    Summary

    Researchers found high-affinity angiotensin II (ANG II) receptors within cell nuclei, suggesting a functional intracellular renin-angiotensin system. This discovery indicates ANG II influences chromatin and gene transcription directly within the cell nucleus.

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    Area of Science:

    • Molecular Biology
    • Endocrinology
    • Biochemistry

    Background:

    • The renin-angiotensin system (RAS) is crucial for regulating blood pressure and fluid balance.
    • While extracellular roles of RAS are well-established, intracellular functions remain less understood.

    Purpose of the Study:

    • To investigate the presence and characteristics of angiotensin II (ANG II) receptors within isolated cell nuclei.
    • To explore the functional implications of nuclear ANG II binding on chromatin structure and transcriptional activity.

    Main Methods:

    • Incubation of isolated rat hepatic and bovine thymus nuclei with radiolabeled ANG II.
    • Displacement assays using varying concentrations of cold ANG II.
    • Analysis of nuclear fragments generated by micrococcal nuclease.

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  • Partial purification of the ANG II binding moiety via DNP gel electrophoresis.

    • Main Results:

    • Displaceable binding of ANG II to nuclear fragments was consistently detected.
    • Nanomolar concentrations of ANG II were sufficient for detectable displacement.
    • Evidence suggests ANG II solubilizes its own receptor, with minimal binding after nuclear digestion.
    • Partial purification of the binding moiety was achieved.

    Conclusions:

    • High-affinity ANG II receptors exist within chromatin.
    • ANG II binding induces conformational changes in chromatin, mimicking enhanced transcriptional activity.
    • These findings support the existence of functional intracellular renin-angiotensin systems.