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Neuropeptide Y distribution in the rat brain.
Science (New York, N.Y.)
|August 26, 1983
Summary
Neuropeptide Y, a peptide from the pancreatic polypeptide family, forms a massive neuronal system in the brain. This peptide is highly concentrated in brain regions like the cortex and hypothalamus.
Area of Science:
- Neuroscience
- Biochemistry
Background:
- Neuropeptide Y (NPY) is a recently isolated peptide belonging to the pancreatic polypeptide family.
- The distribution and function of NPY in the mammalian brain were not fully understood.
Purpose of the Study:
- To investigate the presence and distribution of neuropeptide Y in the mammalian brain.
- To determine if neuropeptide Y is responsible for pancreatic polypeptide-like immunoreactivity.
Main Methods:
- Immunocytochemistry and radioimmunoassay using antibodies to neuropeptide Y.
- Extraction and column chromatography of brain tissue.
- Double immunostaining experiments.
Main Results:
- A widespread neuronal system immunoreactive for neuropeptide Y was identified.
- Neuropeptide Y was most abundant in cortical, limbic, and hypothalamic regions.
- Neuropeptide Y concentrations in the brain exceeded those of all previously discovered peptides.
- Neuropeptide Y was confirmed as the endogenous peptide responsible for pancreatic polypeptide-like immunoreactivity.
Conclusions:
- Neuropeptide Y constitutes a major peptidergic neurotransmitter system in the mammalian brain.
- The high concentration and widespread distribution suggest a significant role for NPY in brain function.
- Neuropeptide Y is the primary source of pancreatic polypeptide-like immunoreactivity in the brain.