[Determination of retinol in human blood plasma using high pressure liquid chromatography]

A quantitative procedure for estimation of retinol in human blood plasma using high pressure liquid chromatography involved separation of the retinoids on an reversed phase analytical column. C19-retinoid aldehyde was used as an internal standard. The procedure enabled to detect up to 0.015 microgram of retinol/ml of blood plasma and may be used for routine clinical analyses.