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Related Experiment Videos

An Amphioxus lanceolatus agglutinating factor for human red cells.

G De Benedictis, P Capalbo

    Journal of Immunogenetics
    |June 1, 1981
    PubMed
    Summary

    Proteins from the lancelet Amphioxus lanceolatus exhibit significant red blood cell agglutinating activity. This factor was identified as an alpha macroglobulin, indicating potential roles in immune responses.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Marine Biology

    Background:

    • Amphioxus lanceolatus (lancelets) are basal chordates with a unique immune system.
    • Humoral factors in invertebrates can play roles in innate immunity.
    • Understanding lancelet immune proteins offers insights into vertebrate immune evolution.

    Purpose of the Study:

    • To investigate the agglutinating activity of humoral proteins from Amphioxus lanceolatus.
    • To characterize the biochemical properties of the identified haemagglutinating factor.

    Main Methods:

    • Assay of agglutinating activity against human red blood cells.
    • Gel filtration chromatography using Sephadex G200.
    • Affinity chromatography on Sepharose ConA.
    • Immunoelectrophoresis using rabbit antiserum.

    Main Results:

    • Humoral proteins from Amphioxus lanceolatus demonstrated considerable agglutinating activity for human red cells.
    • The haemagglutinating factor eluted in the macroglobulin peak (Sephadex G200) and bound to Concanavalin A (ConA) (Sepharose ConA).
    • Immunoelectrophoresis indicated alpha mobility, identifying the factor as an alpha macroglobulin.

    Conclusions:

    • Amphioxus lanceolatus possesses humoral proteins with significant haemagglutinating activity.
    • The active factor is characterized as an alpha macroglobulin, suggesting a conserved immune protein family.
    • This finding contributes to understanding innate immunity in early chordates.

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