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A polymorphic antigen displaying different molecular weights in sheep and cattle sera.
Animal Blood Groups and Biochemical Genetics
|January 1, 1981
Summary
Polymorphic antigen B1 in sheep and cattle sera differs in molecular weight. The active 35,000 MW subunit is conserved across species, while sheep B1 contains an additional 60,000 MW subunit.
Area of Science:
- Immunology
- Veterinary Science
- Biochemistry
Background:
- Polymorphic antigen B1 is found in both sheep and cattle sera.
- This antigen exhibits variations in molecular weight between the two species.
Purpose of the Study:
- To characterize the molecular differences and similarities of polymorphic antigen B1 in sheep and cattle.
- To identify the specific subunits responsible for antigen activity.
Main Methods:
- Purification of antigen B1 from sheep and cattle sera using a specific immunoabsorbent.
- Analysis of molecular weight via SDS polyacrylamide gel electrophoresis and calibrated gel filtration.
Main Results:
- Cattle B1 consists of a single 35,000 MW subunit.
- Sheep B1 comprises both a 35,000 MW subunit and a 60,000 MW subunit.
- The antigen activity resides on the 35,000 MW subunit, which is consistent in both species.
Conclusions:
- The polymorphic antigen B1 shows species-specific variations in subunit composition.
- The functional 35,000 MW subunit of antigen B1 is conserved between sheep and cattle.
- Understanding these molecular differences aids in comparative immunology and diagnostics.