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Histamine release from mast cell granules.

N Chakravarty

    Agents and Actions
    |April 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Calcium ions (Ca2+) trigger histamine release from rat mast cell granules, especially with phosphatidyl serine. This process involves increased calcium uptake and potential changes in granule membrane permeability, offering insights into cellular signaling.

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    Area of Science:

    • Cell Biology
    • Biochemistry
    • Immunology

    Background:

    • Rat peritoneal mast cells are crucial in allergic responses.
    • Mast cell granules store histamine, a key mediator.
    • Understanding histamine release mechanisms is vital for therapeutic development.

    Purpose of the Study:

    • To investigate the role of calcium (Ca2+) and phosphatidyl serine in histamine release from isolated mast cell granules.
    • To explore the effects of various divalent cations on histamine release.
    • To examine calcium uptake by granules during histamine release.

    Main Methods:

    • Isolation of rat peritoneal mast cells and their granules via differential centrifugation and ultrasonic disintegration.
    • Incubation of intact granules with Ca2+ (10 mM) and phosphatidyl serine (25-50 μg/ml).

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  • Assessment of histamine release and calcium uptake using physiological salt solutions and various divalent cations (Mg2+, Ba2+, Sr2+, Ni2+, Mn2+).

    • Main Results:

    • Ca2+ (10 mM) induced rapid histamine release from granules with intact membranes in the presence of phosphatidyl serine.
    • Other divalent cations also facilitated histamine release with phosphatidyl serine.
    • Histamine release triggered by Ca2+ and phosphatidyl serine significantly increased granule calcium uptake, suggesting Ca2+ displaces histamine.

    Conclusions:

    • Calcium ions, particularly in conjunction with phosphatidyl serine, effectively release histamine from mast cell granules.
    • The observed increase in calcium uptake suggests a mechanism where Ca2+ displaces histamine from the granule matrix.
    • Further investigation into how Ca2+ and phosphatidyl serine alter granule membrane permeability is warranted.