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Soluble HLA antigens in normal human immunoglobulin preparations
Tissue Antigens
|March 1, 1982
Summary
Normal human immunoglobulin preparations contain soluble human leukocyte antigens (HLA). Most common HLA antigens were detected, varying with immunoglobulin source and raw materials.
Area of Science:
- Immunology
- Transplantation Science
- Biochemistry
Background:
- Human immunoglobulin (Ig) is a crucial therapeutic agent derived from human plasma.
- Understanding the composition of Ig, including potential immunomodulatory components like HLA antigens, is vital for its safe and effective use.
- Soluble human leukocyte antigens (HLA) are important in immune responses and transplantation.
Purpose of the Study:
- To investigate the presence and profile of soluble HLA antigens in normal human immunoglobulin preparations.
- To compare HLA antigen content in immunoglobulins from different geographical origins (Bulgaria and India).
- To assess the influence of raw material source on soluble HLA antigen levels.
Main Methods:
- Assay of nine lots of normal human immunoglobulin using HLA alloantisera.
- Detection of soluble HLA antigens via inhibition of cytotoxicity.
- Employment of two distinct absorption procedures for enhanced sensitivity.
Main Results:
- Soluble HLA antigens were confirmed in all tested human immunoglobulin preparations.
- Most high-frequency antigens across HLA-A, B, C, and DR loci were detected.
- Significant variation in soluble HLA antigen content was observed between immunoglobulins from Bulgaria and India, with a notable absence of HLA-A3 in Indian samples.
Conclusions:
- Normal human immunoglobulin preparations contain biologically relevant amounts of soluble HLA antigens.
- The composition of soluble HLA antigens in immunoglobulin varies based on geographical origin and production raw materials.
- These findings have implications for understanding the immunomodulatory potential of immunoglobulin therapies.