Biochemical changes in Pinus pinea seeds during storing

Area of Science:

• * Botany
• * Plant Physiology
• * Seed Science

Background:

• * Pinus pinea seeds undergo maturation and potential aging during storage.
• * Understanding biochemical changes is crucial for seed viability.
• * Storage conditions (time and temperature) influence seed quality.

Purpose of the Study:

• * To investigate the effects of storage duration and temperature on Pinus pinea seed germination.
• * To analyze changes in germination inhibitors and biochemical components (proteins, nucleic acids).
• * To determine the optimal storage period for maintaining seed viability.

Main Methods:

• * Freshly harvested Pinus pinea seeds were stored for up to 24 months.
• * Seeds were stored at two different temperatures: room temperature and 4 degrees C.
• * Germination rate, germination inhibitor content, and biochemical differences in soluble proteins and nucleic acids were assessed.

Main Results:

• * Storage for 6-12 months appeared to induce maturation or after-ripening in Pinus pinea seeds.
• * Longer storage periods (beyond 12 months) initiated seed aging processes.
• * Aging seeds showed reduced germination rates and altered biochemical profiles, including decreased protein and nucleic acid levels during germination.

Conclusions:

• * Pinus pinea seed maturation can occur during the initial months of storage.
• * Extended storage promotes seed aging, negatively impacting germination and biochemical potential.
• * Optimal storage duration is critical to balance maturation benefits and prevent aging-induced degradation.