Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular weight determination program.

C V Maina, G P Nolan, A A Szalay

    Nucleic Acids Research
    |January 11, 1984
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a computer program for calculating molecular weights of DNA, RNA, and proteins after gel electrophoresis. The software uses reference molecule data to accurately determine the size of unknown biological molecules.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Extra-Islet Pancreas Supports Autoimmunity in Human Type 1 Diabetes.

    medRxiv : the preprint server for health sciences·2023
    Same author

    Mass cytometry identifies a distinct monocyte cytokine signature shared by clinically heterogeneous pediatric SLE patients.

    Journal of autoimmunity·2017
    Same author

    The histone deacetylase inhibitor givinostat (ITF2357) exhibits potent anti-tumor activity against CRLF2-rearranged BCP-ALL.

    Leukemia·2017
    Same author

    Mass cytometry analysis reveals hyperactive NF Kappa B signaling in myelofibrosis and secondary acute myeloid leukemia.

    Leukemia·2016
    Same author

    Single timepoint models of dynamic systems.

    Interface focus·2014
    Same author

    Generation of high-titer, helper-free retroviruses by transient transfection.

    Methods in molecular medicine·2014
    Same journal

    Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

    Nucleic acids research·2026
    Same journal

    Correction to 'Differentiable partition function calculation for RNA'.

    Nucleic acids research·2026
    Same journal

    Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

    Nucleic acids research·2026
    Same journal

    Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

    Nucleic acids research·2026
    Same journal

    Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

    Nucleic acids research·2026
    Same journal

    Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

    Nucleic acids research·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Computational Biology

    Background:

    • Gel electrophoresis is a standard technique for separating biomolecules by size.
    • Accurate molecular weight determination is crucial for analyzing DNA, RNA, and proteins.
    • Manual measurement and calculation of molecular weights can be time-consuming and prone to error.

    Purpose of the Study:

    • To develop and describe a computer program for automating molecular weight determination.
    • To provide a user-friendly tool for analyzing gel electrophoresis data.
    • To enhance the precision and efficiency of molecular size analysis.

    Main Methods:

    • A computer program was developed using Apple Pascal for the Apple II Plus.
    • The program utilizes migration distances of known molecules in a reference lane.

    Related Experiment Videos

  • It computes a best-fit polynomial equation (second or third order) to the data points.
  • Migration distances are input via an analog tablet.

    • Main Results:

    • The program accurately determines the molecular weight of DNA, RNA, and protein molecules.
    • It calculates the sizes of unknown molecules based on the generated calibration curve.
    • The system provides a quantitative analysis of biomolecule sizes from gel electrophoresis.

    Conclusions:

    • The developed computer program offers an efficient and accurate method for molecular weight determination.
    • This tool simplifies the analysis of gel electrophoresis results in molecular biology research.
    • Automation of molecular weight calculation improves data reliability and experimental throughput.