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Related Experiment Videos

A human alloreactive inducer T cell clone that selectively activates antigen-specific suppressor T cells.

N Mohagheghpour, N K Damle, D K Moonka

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1984
    PubMed
    Summary

    Researchers identified a specific T cell clone (SP-21) that activates antigen-specific suppressor cells. This clone, derived from Leu-3+,8+ T cells, selectively induces Leu-2+ suppressor cells without direct suppression or cytotoxicity.

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    Area of Science:

    • Immunology
    • Cell Biology
    • T cell immunology

    Background:

    • T cells with Leu-3+,8+ phenotype are crucial for inducing antigen-specific Leu-2+ suppressor cells.
    • Cyclosporin A (CsA) in mixed lymphocyte reactions promotes the activation of suppressor-inducer Leu-3+,8+ cells.

    Purpose of the Study:

    • To generate a T cell clone with specific suppressor-inducer properties.
    • To investigate the functional repertoire of such a T cell clone.

    Main Methods:

    • Activation of Leu-3+,8+ T cells with allogeneic (HLA-DR4+) lymphocytes in the presence of CsA.
    • Propagation of activated T cells with IL-2 containing conditioned medium to derive clone SP-21.
    • Co-culture of clone SP-21 with autologous Leu-2+ cells and assessment of suppressor cell activation.

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    Main Results:

    • Clone SP-21, a noncytotoxic, nonsuppressor clone, specifically proliferates to HLA-DR4+ antigen.
    • Clone SP-21 selectively activates Leu-2+ suppressor cells in the presence of autologous Leu-2+ cells (without HLA-DR4+ cells).
    • Activated Leu-2+ suppressor cells inhibit the response of Leu-3+ cells to DR4+ stimulator cells.

    Conclusions:

    • A T cell clone (SP-21) with a narrow functional repertoire possesses the necessary signals to activate antigen-specific suppressor cells.
    • This clone demonstrates a specific pathway for inducing suppressor T cell activity without direct cytotoxic or helper functions.