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Lymphokine-induced suppressor B cells.

K M Gilbert, M K Hoffmann

    Immunology
    |July 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Murine B cell antibody production involves macrophage and T-cell lymphokines. Suppressor B cells, induced by IL-1 and (DL) TRF, impair antibody responses when T-cell-derived regulatory factor (TRF) is added early.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Antibody production by B cells requires accessory signals from macrophages and T helper cells.
    • Lymphokines, including IL-1 and T-cell-replacing factor (TRF), mediate these accessory activities.
    • The timing of TRF addition influences B cell responses, suggesting complex regulatory mechanisms.

    Purpose of the Study:

    • To investigate the mechanisms underlying suboptimal helper activity observed when TRF is added early during in vitro antibody production.
    • To identify the cell type and inducing factors responsible for the generation of suppressor cells.
    • To elucidate the distinct pathways involved in B cell activation versus suppressor B cell induction.

    Main Methods:

    • In vitro culture of murine B cells with specific lymphokines (IL-1, IL-2, TRF, (DL) TRF).

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  • Assessment of antibody production and cell surface phenotype analysis (Thy 1, Ia, IgG, IgD) of suppressor cells.
  • Serological examination to characterize the phenotype of helper factor-induced suppressor cells.

    • Main Results:

    • Early addition of TRF leads to suboptimal antibody production due to the generation of suppressor B cells, not mediator inactivation.
    • These suppressor B cells exhibit a B cell surface phenotype (Thy 1-, Ia+, IgG+, IgD+).
    • Distinct induction pathways exist: IL-1 and IL-2 for antibody-forming cells, and IL-1 and (DL) TRF for suppressor B cells.

    Conclusions:

    • The generation of suppressor B cells is a critical regulatory mechanism impacting antibody production.
    • The findings reveal a dichotomy in the induction pathways for effector B cells and suppressor B cells.
    • Interleukin-2 (IL-2) and a factor in TRF, designated (DL) TRF, play differential roles in B cell activation and suppression.