Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Long-term suppressor cell lines. I. Demonstration of suppressive function.

J D Levich, W O Weigle, D E Parks

    European Journal of Immunology
    |December 1, 1984
    PubMed
    Summary

    Suppressor T cell lines, initially antigen-specific, gain broader regulatory function with prolonged culture. However, antigen-specific suppressor T cells can still be isolated and retain their original specificity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Role of polyclonal B-cell activation in self/non-self discrimination.

    Immunology today·2014
    Same author

    Cytokines in the induction and circumvention of peripheral tolerance.

    Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research·1999
    Same author

    Evidence for an underlying CD4 helper and CD8 T-cell defect in B-cell-deficient mice: failure to clear persistent virus infection after adoptive immunotherapy with virus-specific memory cells from muMT/muMT mice.

    Journal of virology·1998
    Same author

    CD4+ T-cell subsets and cytokines involved in peripheral tolerance.

    Immunology today·1997
    Same author

    CD40 ligand expressed on B cells in the BXSB mouse model of systemic lupus erythematosus.

    Journal of immunology (Baltimore, Md. : 1950)·1997
    Same author

    Advances in basic concepts of autoimmune disease.

    Clinics in laboratory medicine·1997

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Suppressor T cells (Ts) play a crucial role in immune regulation.
    • Established Ts lines are valuable tools for studying immune tolerance and suppression.
    • Antigen specificity is a hallmark of many immune responses, including T cell-mediated suppression.

    Purpose of the Study:

    • To investigate the long-term behavior of suppressor T cell lines in culture.
    • To characterize changes in the functional properties of Ts cells upon prolonged culture.
    • To determine if antigen-specific Ts cells can be isolated from lines with altered specificity.

    Main Methods:

    • Generation and maintenance of Ts cell lines from mouse spleens.
    • Assays to measure suppression of lymph node cell proliferation and spleen cell antibody production.

    Related Experiment Videos

  • Antigen-binding assays using human gamma globulin (HGG)-coated plates.

    • Main Results:

    • Prolonged culture of Ts lines led to increased suppressive activity.
    • Ts cell lines acquired the ability to suppress responses to antigens other than their original target (HGG), indicating polyclonal activation.
    • HGG-specific Ts cells were isolated by HGG-binding, and these cells retained HGG-specific suppression.

    Conclusions:

    • Long-term culture of Ts cells can lead to a loss of strict antigen specificity.
    • Despite polyclonal activation, antigen-specific Ts cells persist within the lines and can be functionally isolated.
    • These findings have implications for understanding immune regulation and developing cell-based therapies.