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Related Experiment Videos

Long-term suppressor cell lines. II. Suppressor-target interactions.

J D Levich, W O Weigle, D E Parks

    European Journal of Immunology
    |December 1, 1984
    PubMed
    Summary

    Long-term suppressor T cell (Ts) lines exhibit genetic restrictions in suppressing lymph node cell proliferation, mapping to the major histocompatibility complex's I region. These Ts cells primarily target helper T cells for suppression.

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    Area of Science:

    • Immunology
    • Cellular immunology
    • T cell biology

    Background:

    • Long-term cultured murine suppressor T cell (Ts) lines demonstrate genetically restricted suppression of primed lymph node cells (LNC).
    • Previous studies indicated Ts cell activity is influenced by the major histocompatibility complex (MHC).

    Purpose of the Study:

    • To characterize the genetic basis and cellular targets of suppressor T cell activity in long-term cultured lines.
    • To investigate the role of MHC in Ts cell-mediated suppression.

    Main Methods:

    • Culturing murine Ts cell lines for over one year.
    • Assessing suppression of LNC proliferation.
    • Mapping genetic restriction to the MHC I region (I-A, I-E, or I-J loci).
    • Irradiation of Ts cells to assess activity.

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  • Co-culture experiments with primed B cells and cloned helper T cells to identify target cells.

    • Main Results:

    • Genetic restriction of Ts cell suppression maps to the I region of the MHC.
    • Suppressive activity is minimally affected by 2000 rads of irradiation.
    • Helper T cells are identified as the primary target of Ts cell-mediated suppression.
    • Suppression is most effective when Ts cells are introduced early in the culture assay.
    • Ts cells suppress the anti-human gamma globulin plaque-forming cell response mediated by helper T cells.

    Conclusions:

    • Long-term Ts cell lines maintain genetically restricted suppressive function.
    • The MHC I region, specifically I-A, I-E, or I-J, is crucial for this genetic restriction.
    • Helper T cells are the direct targets of suppressor T cell activity.
    • These findings contribute to understanding T cell-mediated immune regulation and MHC restriction.