Related Experiment Video
Updated: Aug 19, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Published on: January 31, 2018
Distribution of S1-nuclease sensitive sites and double-strand breaks in gamma-irradiated lambda-DNA
Abstract:
Double-strand breaks and regions with unpaired bases (S1-nuclease sensitive sites) are formed in the DNA of gamma-irradiated lambda-phages. The frequency of both events was determined in the various fragments produced after digestion with a restriction enzyme (Eco RI). The results show that both types of radiation damage are randomly distributed in the DNA molecule. There is no hint that the S1 sites are formed in preferred regions of the lambda-genome.
Related Concept Videos
Nucleotide Excision Repair
Fixing Double-strand Breaks
Homologous Recombination
Single-Strand DNA Binding Proteins
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Fixing Double-strand Breaks

