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Updated: Aug 31, 2026

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
Published on: November 10, 2010
[Immunochemical characteristics of a factor causing antigen-specific lysis of thymus-dependent lymphocytes]
Abstract:
A factor capable of lysing in vitro, in the presence of a specific antigen, the cells of the lymph nodes and the thymus of intact mice was revealed in the supernatant obtained after the centrifugation of a suspension of viable cells of the lymph nodes and the thymus of the immunized mice. It was found by immunochemical methods that this factor had a mol. wt. of about 30000 dalton and an electrophoretic mobility in polyacrylamide gel exceeding that of mouse blood serum albumin. Besides, it was revealed by the precipitation reaction in agar that it was not an immunoglobulin or its chains.
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