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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
Published on: October 30, 2016
Natural killer cell activity associated with reticulum cell neoplasms
International Journal of Cancer
|March 15, 1980
Summary
Natural killer (NK) cell activity significantly increases in mice with growing reticulum-cell neoplasm (RCN). This augmented NK activity targets various tumor cells, suggesting RCN may uniquely promote NK responses.
Area of Science:
- Immunology
- Oncology
Background:
- Natural killer (NK) cells are crucial for innate immunity against tumors.
- Reticulum-cell neoplasm (RCN) is a type of lymphoid malignancy.
Purpose of the Study:
- To investigate the relationship between RCN growth and NK cell activity in SJL/J mice.
- To characterize the properties of NK cells involved in the response to RCN.
Main Methods:
- Assessing NK activity in lymphoid cells from RCN-bearing mice.
- Characterizing NK cell properties (adherence, phagocytosis, resistance to X-ray and antibodies).
- Evaluating NK cell cytotoxicity against various tumor targets and the effect of RCN extracts.
Main Results:
- A marked increase in NK activity was observed in SJL/J mice with growing RCN.
- NK cells were non-adherent, non-phagocytic, X-ray resistant, and partially resistant to anti-Thy 1.2 serum.
- NK activity was selective for tumor targets and increased with RCN growth, but not with other tumors.
- NK cell origin was confirmed as host-derived in (C57BL/6 X SJL/J)F1 mice.
Conclusions:
- RCN growth is closely associated with elevated NK activity in SJL/J mice.
- RCN may possess a unique mechanism for promoting NK cell activity.
- Further research is needed to elucidate the cellular interactions involved.
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