Related Experiment Videos
An experimental approach to energy balance in a microbial process using the dichromate oxidation method
Zeitschrift Fur Allgemeine Mikrobiologie
|January 1, 1981
Summary
This study quantifies energy in Bordetella pertussis cultures using dichromate oxidation to determine reduction equivalents and caloric values, establishing a complete energy balance for microbial growth.
Area of Science:
- Microbiology
- Biochemistry
- Bioenergetics
Background:
- Understanding microbial energy metabolism is crucial for optimizing bioprocesses.
- Accurate quantification of energy transformation in bacterial cultures is essential for metabolic studies.
Purpose of the Study:
- To determine reduction equivalents of organic substrates, cell mass, and extracellular products in Bordetella pertussis cultivations.
- To establish a complete energy balance for aerobic microbial processes.
Main Methods:
- Dichromate oxidation method for quantifying reduction equivalents.
- Transformation of reduction equivalents into caloric values.
- Application of the law of conservation of energy for energy balance.
Main Results:
- Quantified reduction equivalents for organic substrates, cell mass, and extracellular products.
- Converted reduction equivalents to caloric values.
- Developed a complete energy balance model for aerobic microbial cultivation.
Conclusions:
- The dichromate oxidation method provides a reliable way to assess energy transformations in bacterial cultures.
- A comprehensive energy balance can be achieved by integrating caloric values derived from reduction equivalents.
- This approach is applicable to aerobic microbial processes like Bordetella pertussis cultivation.