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[Structure containing cellular mRNA in picornavirus infections]
Molekuliarnaia Biologiia
|September 1, 1981
Summary
Cellular messenger RNA (mRNA) forms an inactive 100S structure during encephalomyocarditis virus infection. However, isolated RNA from this structure remains functional for protein synthesis.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Context:
- Investigating cellular mRNA fate during viral infection is crucial for understanding host-pathogen interactions.
- Krebs-2 ascites carcinoma cells provide a model system for studying viral effects on mammalian cells.
Purpose:
- To determine the state and function of cellular mRNA during encephalomyocarditis virus (EMC) infection.
- To characterize the novel 100S structure containing cellular mRNA.
Summary:
- Cellular mRNA in infected Krebs-2 cells aggregates into a 100S structure with a buoyant density of 1.50--1.519 g/cm3.
- This 100S structure is translationally inactive, meaning it does not directly produce proteins.
- RNA isolated from the 100S structure is functionally active in cell-free protein synthesis systems.
Impact:
- Reveals a mechanism of mRNA sequestration during viral infection.
- Suggests a potential regulatory role for the 100S structure in viral pathogenesis.
- Provides insights into mRNA processing and translation control in virus-infected cells.