Activation of calmodulin-dependent NAD+ kinase by trypsin

Sea urchin egg NAD+ kinase (ATP:NAD+ 2'-phosphotransferase, EC 2.7.1.23), a calmodulin-dependent enzyme, can be activated by a moderate treatment with trypsin in a similar fashion to calmodulin. Stimulation by trypsin is dependent on its concentration (half-maximal dose: 1.5 microgram/ml) but independent of the presence of calcium. This suggests that limited proteolysis is able to activate NAD+ kinase as described for several other calmodulin-activated enzymes and that these enzymes may interact with calmodulin in a similar way.