Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Is Percoll innocuous to cells?

J S Wakefield, J S Gale, M V Berridge

    The Biochemical Journal
    |March 15, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The link between cutaneous inflammation and cognitive impairment.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2022
    Same author

    The cellular and molecular toxicity of sporidesmin.

    New Zealand veterinary journal·2020
    Same author

    The Rev. Mr. Gale, on the Cow-Pox.

    The Medical and physical journal·2018
    Same author

    Effects of sporidesmin on cultured biliary tract cells from Romney lambs that differed in their sensitivity to sporidesmin.

    New Zealand veterinary journal·2018
    Same author

    Mitochondrial transfer between cells: Methodological constraints in cell culture and animal models.

    Analytical biochemistry·2017
    Same author

    Survival of Neisseria meningitidis outside of the host: environmental effects and differences among strains.

    Epidemiology and infection·2017
    Same journal

    Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

    The Biochemical journal·2026
    Same journal

    Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

    The Biochemical journal·2026
    Same journal

    Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

    The Biochemical journal·2026
    Same journal

    Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

    The Biochemical journal·2026
    Same journal

    Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

    The Biochemical journal·2026
    Same journal

    A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

    The Biochemical journal·2026
    See all related articles

    Percoll particles are ingested by macrophages, Kupffer cells, and Leydig cells, affecting cell adherence. This density medium may impact cell surface interactions and functions.

    Area of Science:

    • Cell biology
    • Immunology
    • Biochemistry

    Background:

    • Percoll is a density gradient medium used for cell and organelle separation.
    • Immune cells like macrophages and Kupffer cells play crucial roles in host defense.
    • Leydig cells are vital for testosterone production in the testes.

    Purpose of the Study:

    • To investigate the interaction of Percoll particles with specific cell types.
    • To assess the effect of Percoll exposure on macrophage adherence.
    • To explore potential mechanisms underlying Percoll's impact on cell surface properties.

    Main Methods:

    • Isolation of peritoneal macrophages (mice), Kupffer cells (rats), and Leydig cells (rats).
    • Exposure of isolated cells to Percoll particles.

    Related Experiment Videos

  • Assessment of cell adherence to plastic surfaces.
  • Evaluation of Percoll ingestion by cells at different temperatures.

    • Main Results:

    • Macrophages, Kupffer cells, and Leydig cells ingested significant amounts of Percoll particles.
    • Percoll exposure reduced macrophage adherence to plastic, even at low temperatures.
    • Percoll's effect on adherence may involve transient cell surface interactions.

    Conclusions:

    • Percoll particles are readily ingested by key immune and endocrine cells.
    • Percoll can negatively impact macrophage adherence, suggesting cell surface alterations.
    • Further research is needed to understand the broader implications of Percoll on cell function.