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Colorimetric assay for aminoacyl-tRNA synthetases
Analytical Biochemistry
|April 1, 1983
Abstract:
A simple, rapid, and inexpensive procedure for the determination of aminoacyl-tRNA synthetase activity is described. The assay is based on a green color formed between PPi and ammonium molybdate in the presence of mercaptoethanol. The sensitivity is in the nanomole range and is comparable with the conventional [32P]PPi-ATP exchange assay. Amino acid, ATP, magnesium ion, and most common reagents do not interfere with the color yield.