The heptapeptide Met-enkephalin-Arg6, Phe7 is released from rat striatum in vitro by high potassium

We present evidence that the heptapeptide Met-enkephalin-Arg6, Phe7 is released from rat striatal slices following depolarization by a high concentration of KCl. The heptapeptide-immunoreactive material released in the incubation medium, which is shown by HPLC and radioimmunoassay of serial dilutions to represent authentic heptapeptide, is detectable in the incubation media only in the presence of a cocktail of peptidase inhibitors containing thiorphan (0.1 microM), captopril (1 microM) and bestatin (20 microM).