Related Experiment Video
Updated: Aug 14, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
Mathematical modelling of stimulus-secretion coupling in the pancreatic B-cell. I. Dynamics of insulin release
Abstract:
A mathematical model for cyclic AMP-Ca2+ interactions in pancreatic islets, defined in a previous study, was modified to include two new features. First, the cytosol and vacuolar Ca2+ pools were stratified in a cortical layer (representing 1 to 20% of the total cellular pool) and central core. Second, the changes in Ca2+ inflow and Ca2+ fractional outflow rate evoked by a rise in glucose concentration were discontinued during the 3rd and 4th min of stimulation. In this onion leaf sheet model, the biphasic pattern of glucose-induced insulin release could be simulated. This new model emphasizes the significance of both cytosol heterogeneity and signal discontinuity in the dynamics of insulin secretion.
Related Concept Videos
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Insulin Secretory Vesicles
Regulation of Hormone Secretion
Humoral stimuli,...
Cells and Secretions of the Pancreas
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
Insulin and C-peptide are co-secreted in...

