[Phosphorescence quenching in evaluation of the steric interactions of heme-containing proteins]

The rate constants of exchange phosphorescence quenching of eosin by ferricyanide, hemin and hem-proteins (cytochrome c, myoglobin , hemoglobin, leghemoglobin) are measured. The steric factor of hem of cytochrome c is evaluated from the constant of eosin phosphorescence quenching by cytochrome c.