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Cellular DNA region involved in induction of thymic lymphomas (Mlvi-2) maps to mouse chromosome 15
Abstract:
Two cellular DNA regions representing common domains for proviral DNA integration ( Mlvi -1 and Mlvi -2) have been identified in Moloney murine leukemia virus-induced rat thymic lymphomas. Cellular sequences which were free of repeated DNA derived from a clone that defines the Mlvi -2 integration domain (lambda Cl228 ) were found to be highly conserved in a variety of vertebrate species that we examined, including mice, hamsters, cats, and humans. In this study, we identified the chromosomal map location of the Mlvi -2 homologous sequences in mice by using hamster-mouse somatic cell hybrids. The results show that Mlvi -2 is present on mouse chromosome 15 but is unrelated to the c-myc and c-sis proto-oncogenes, which map on the same chromosome. Since aberrations on chromosome 15 have been observed reproducibly in mouse thymomas, our data suggest that Mlvi -2 may define a novel sequence involved in the induction or progression of murine thymic lymphomas.
Insights
Moloney murine leukemia virus integration site 2 (Mlvi-2) sequences are conserved across vertebrates. This study mapped Mlvi-2 to mouse chromosome 15, suggesting its potential role in murine thymic lymphoma development.
Area of Science:
- * Molecular biology
- * Virology
- * Genetics
Background:
- * Moloney murine leukemia virus induces rat thymic lymphomas.
- * Common proviral DNA integration domains include Mlvi-1 and Mlvi-2.
- * Mlvi-2 defining sequences are conserved across diverse vertebrate species.
Purpose of the Study:
- * To determine the chromosomal location of Mlvi-2 homologous sequences in mice.
- * To investigate the relationship between Mlvi-2 and other proto-oncogenes on the same chromosome.
Main Methods:
- * Hamster-mouse somatic cell hybrid analysis was used to map Mlvi-2.
- * Comparative sequence analysis was performed.
Main Results:
- * Mlvi-2 was mapped to mouse chromosome 15.
- * Mlvi-2 is not related to the c-myc or c-sis proto-oncogenes.
- * Chromosome 15 aberrations are linked to mouse thymomas.
Conclusions:
- * Mlvi-2 may represent a novel sequence involved in murine thymic lymphoma induction or progression.
- * Further research is warranted to elucidate the specific role of Mlvi-2 in lymphomagenesis.