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Gene transfer into mammalian cells.
Developments in Biological Standardization
|January 1, 1983
Summary
Introducing purified genes into animal cells aids gene expression studies and creates genetically modified cell lines. Methods like transfection and micro-injection enable transient or permanent foreign gene expression.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Gene expression and regulation studies are crucial in molecular and cell biology.
- Genetically modified cell lines are essential tools for biological research.
Purpose of the Study:
- To review methods for introducing purified genes into animal cells.
- To discuss the establishment of genetically modified cell lines.
- To explore gene expression and regulation studies.
Main Methods:
- DNA transfer into higher eukaryotic cells.
- Calcium phosphate-mediated transfection.
- Cell fusion with liposomes or bacterial protoplasts.
- Micro-injection of DNA.
Main Results:
- Transient and/or permanent expression of foreign genes is achievable.
- Successful establishment of biochemically transformed cells.
Conclusions:
- Various DNA transfer methods facilitate the study of gene expression.
- Genetically modified cell lines can be reliably established using these techniques.