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Related Experiment Videos

Candidal antigen detection in pulmonary candidiasis.

D M Humphrey, M H Weiner

    The American Journal of Medicine
    |April 1, 1983
    PubMed
    Summary

    Immunohistochemical analysis of lung tissue improves diagnosis of invasive pulmonary candidiasis. Detecting candidal antigens in lung samples and blood helps distinguish fungal pneumonia from other lung infections.

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    Coexistent oral cryptococcosis and Kaposi's sarcoma in acquired immunodeficiency syndrome.

    Cutis·1992

    Area of Science:

    • Medical Mycology
    • Pulmonology
    • Pathology

    Background:

    • Pulmonary candidiasis diagnosis can be challenging.
    • Distinguishing invasive fungal pneumonia from colonization or contamination is critical.

    Purpose of the Study:

    • To characterize invasive pulmonary candidiasis using immunohistochemical analysis.
    • To assess the diagnostic utility of detecting candidal antigens in lung tissue.

    Main Methods:

    • Immunohistochemical analysis of lung tissue samples from 58 hospital subjects using antiserums to candidal mannan and cytoplasmic antigens.
    • Detection of antigenemia using mannan radioimmunoassay in serum samples.

    Main Results:

    • Invasive pulmonary candidiasis cases showed abundant fungal antigens in lung specimens with distinct immunohistologic patterns.
    • Antigenemia was detected in patients with invasive pulmonary candidiasis.
    • Minimal antigens were found in non-invasive cases, and none in non-candidal pneumonias.
    • Immunohistochemical detection significantly increased diagnostic yield compared to routine stains.

    Conclusions:

    • Immunohistochemical detection of candidal antigens facilitates diagnosis of candidal pneumonia.
    • This method distinguishes invasive pulmonary candidiasis from other conditions like aspiration or contamination.

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