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Morphometric measurement of cellular hypertrophy.

A V Loud, G Olivetti, P Anversa

Laboratory Investigation; a Journal of Technical Methods and Pathology

|August 1, 1983

Summary

The point-counting method for measuring cellular hypertrophy is unreliable. A more accurate method involves assessing cell size increase per nucleus, especially in diverse cell types like cardiac myocytes.

Area of Science:

Cell Biology
Histology
Quantitative Morphology

Background:

Cellular hypertrophy is a critical indicator of tissue growth and adaptation.
Accurate measurement of cellular hypertrophy is essential for understanding physiological and pathological processes.
Existing morphometric techniques vary in their reliability and accuracy.

Purpose of the Study:

To compare the reliability of two morphometric methods for quantifying mean cellular hypertrophy.
To evaluate the accuracy of the point-counting technique versus nuclear profile counting.
To determine if mean nuclear volume remains constant during cellular growth.

Main Methods:

Comparison of a point-counting technique (nuclear to cytoplasmic ratios) with nuclear profile counting in tissue sections of varying thickness.

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Measurement of cellular hypertrophy in 18 normal and 18 induced growth examples.

Inclusion of five cell types: cardiac myocytes, aortic smooth muscle cells, capillary endothelium, glomerular mesangial cells, and epithelial cells.

Main Results:

Little agreement was observed between the two morphometric methods.
Mean nuclear hypertrophy showed wide and unpredictable variations (+/- 20% in most populations).
The point-counting method demonstrated significant unreliability for quantitative hypertrophy assessment.

Conclusions:

The point-counting technique alone is not a reliable quantitative measure of cellular hypertrophy.
Nuclear profile counting, utilizing greater tissue sampling, offers a more accurate assessment.
Cellular hypertrophy is better defined by cell size increase per nucleus than by volume ratios.