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RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance
Molecular & General Genetics : MGG
|January 1, 1983
Abstract:
Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.