Artifactual hydropic degeneration in skin biopsy specimens immersed in saline: a light and electron microscopic study

An artifact, consisting of hydropic degeneration of the basal cells and subepidermal bulla formation, is described in skin-punch biopsy specimens immersed in normal saline. The histologic appearance and ultrastructural features of this artifact are discussed. Proper handling of skin specimens intended for immunofluorescent studies is stressed. Similarities are noted between the histologic and ultrastructural findings of this artifact and of epidermolysis bullosa simplex and pathologic hydropic degeneration, as is seen in lupus erythematosus.