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Related Experiment Videos

Invasion in vitro. Methods of analysis.

M M Mareel

    Cancer Metastasis Reviews
    |January 1, 1983
    PubMed
    Summary

    Assessing tumor invasion requires robust methods. Current in vitro assays show promise, but further development is needed for accurate quantification of invasion mechanisms.

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    Area of Science:

    • Oncology
    • Cell Biology
    • Biophysics

    Background:

    • Understanding tumor invasion mechanisms is crucial for cancer research.
    • In vitro assays are developed to study interactions between invasive cells and host tissues.
    • Evaluating tumor invasiveness in vitro presents significant methodological challenges.

    Purpose of the Study:

    • To review and discuss methods for judging and quantifying tumor invasion in vitro.
    • To highlight the limitations and potential of current techniques in studying invasion kinetics and cell-matrix interactions.
    • To emphasize the need for improved analytical and quantitative methods for invasion assessment.

    Main Methods:

    • Histology for evaluating tumor invasiveness in vivo.
    • Time-lapse cinematography for studying kinetic aspects of invasion.
    • Electron microscopy for detailed cell-matrix interactions.
    • Two-compartment assays for isolating highly invasive cells.
    • Radiolabeling assays to assess cytotoxic effects and extracellular matrix lysis.

    Main Results:

    • Histology is a standard for in vivo assessment but lacks in vitro correlation.
    • Electron microscopy provides insights into cell-matrix interactions.
    • Two-compartment assays can isolate highly invasive and metastatic cell subpopulations.
    • Lytic effects on the extracellular matrix by invasive cells can be measured via radioactivity.

    Conclusions:

    • No single generally accepted method exists for quantifying in vitro invasion.
    • Further research is essential to develop better methods for analyzing and quantifying tumor invasion.
    • Corroborative analysis using multiple methods is currently the most reliable approach.

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