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Related Experiment Videos

Studies on B-cell activation in vitro.

F Melchers, C Corbel

    Annales D'Immunologie
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    T-cell-derived factors are crucial for B-cell activation, proliferation, and maturation into antibody-secreting cells. Accessory cells and soluble factors from macrophages and T cells are essential for restoring B-cell responsiveness, challenging non-specific activation models.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • T cells provide essential factors for B cell activation, proliferation, and maturation into immunoglobulin (Ig)-secreting cells.
    • T-cell lines and hybridomas can produce these B-cell stimulating factors upon antigen or mitogen stimulation.
    • The production of these factors by T-cell hybridomas can be unstable, even after recloning.

    Purpose of the Study:

    • To investigate the role of accessory cells and soluble factors in T-cell-dependent B cell activation.
    • To explore the mechanisms underlying mitogen-induced B cell responsiveness.
    • To challenge the single, non-specific signal model of B cell activation.

    Main Methods:

    • Generation and stimulation of T-cell lines and hybridomas.
    • B cell activation assays with and without accessory cells or soluble factors.

    Related Experiment Videos

  • Mitogen responsiveness assays on spleen B cell populations.

    • Main Results:

    • T-cell hybridomas produce B-cell replication and maturation factors, but this production can be unstable.
    • Mitogen responsiveness of B cells is abolished by cell depletion but can be restored by accessory cells (e.g., peritoneal cells, macrophages) or soluble factors.
    • Restoration of responsiveness occurs with factors from activated macrophages and T cells.

    Conclusions:

    • B cell activation requires T-cell help and is influenced by accessory cells and soluble factors.
    • Mitogenic activation of B cells is not mediated by a single, non-specific signal.
    • B cell activation is dependent on T cells but not macrophages.