Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Demonstration of circulating immune complexes using a C1q-solid phase enzyme immunoassay].

W Schössler, F Hiepe, K Courault

    Biomedica Biochimica Acta
    |January 1, 1983
    PubMed
    Summary

    A new enzyme-immunoassay accurately measures circulating immune complexes in human serum. This sensitive and specific method aids in diagnosing conditions like systemic lupus erythematosus.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Refractory mixed proliferative and membranous lupus nephritis treated with the topoisomerase I inhibitor irinotecan as add-on therapy.

    Scandinavian journal of rheumatology·2021
    Same author

    [Autologous hematopoietic stem cell transplantation for autoimmune diseases : Current indications and mode of action, a review on behalf of the EBMT Autoimmune Diseases Working Party (ADWP)].

    Zeitschrift fur Rheumatologie·2020
    Same author

    [Autologous hematopoietic stem cell transplantation for systemic sclerosis : Position statement of the stem cell therapy working party of the German Society of Rheumatology].

    Zeitschrift fur Rheumatologie·2020
    Same author

    [Autologous hematopoietic stem cell transplantation in systemic lupus erythematosus].

    Zeitschrift fur Rheumatologie·2016
    Same author

    Long-term follow-up of fertility and pregnancy in autoimmune diseases after autologous haematopoietic stem cell transplantation.

    Rheumatology international·2016
    Same author

    Autoantibodies, complement and type I interferon as biomarkers for personalized medicine in SLE.

    Lupus·2016

    Area of Science:

    • Immunology
    • Biochemistry
    • Clinical Chemistry

    Context:

    • Circulating immune complexes (CICs) are implicated in various autoimmune diseases.
    • Accurate quantification of CICs is crucial for diagnosis and monitoring disease activity.
    • Existing methods for CIC detection may lack sensitivity or specificity.

    Purpose:

    • To describe a novel enzyme-immunoassay (EIA) for the quantitative determination of CICs in human serum.
    • To validate the sensitivity and specificity of the developed EIA using aggregated IgG standards.
    • To present preliminary data on CIC levels in normal individuals and patients with systemic lupus erythematosus (SLE).

    Summary:

    • A sensitive enzyme-immunoassay utilizing alkaline phosphatase-conjugated anti-human IgG and solid-phase C1q has been developed for quantifying CICs.

    Related Experiment Videos

  • The assay demonstrates a detection range from 4 ng/ml to 60 µg/ml of aggregated IgG.
  • Initial results indicate the method's specificity and sensitivity for detecting CICs in human serum.

    • Impact:

    • Provides a reliable tool for the clinical laboratory to assess CIC levels.
    • May improve the diagnosis and management of immune complex-mediated diseases, such as SLE.
    • Offers a potentially more accessible and accurate method for CIC detection compared to existing techniques.