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The human hypothalamic LHRH precursor is the same size as that in rat and mouse hypothalamus
Biochemical and Biophysical Research Communications
|December 28, 1983
Abstract:
The synthesis of the decapeptide luteinizing hormone releasing hormone (LHRH) in human, rat and mouse brain has been investigated by studying the in vitro translation products of Poly A+ mRNA extracts from the hypothalamus. The translation products of all three species contained a single 28000 MW polypeptide which immunoprecipitated with a specific anti-LHRH serum. This polypeptide was not present in the translation products of Poly A+ mRNA extracts from the hypothalamus of the hypogonadal mouse, a mutant strain totally deficient in LHRH. These results show that in the human, rat and normal mouse, LHRH is synthesized as a component of a precursor peptide with a molecular weight of 28000.