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Related Experiment Videos

Chromosome mapping in cattle using mouse myeloma/calf lymph node cell hybridomas.

A R Dain, E M Tucker, R A Donker

    Biochemical Genetics
    |June 1, 1984
    PubMed
    Summary

    This study mapped bovine enzyme genes in hybrid cells. Bovine peptidase C (PEP C) was assigned to chromosome 5, and lactate dehydrogenase B/peptidase B (LDH B/PEP B) to chromosome 19.

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    Measurement of [Ca2+] using the Fluorometric Imaging Plate Reader (FLIPR).

    Methods in molecular biology (Clifton, N.J.)·1999

    Area of Science:

    • Genetics
    • Biochemistry
    • Cell Biology

    Background:

    • Gene mapping is crucial for understanding genetic organization and disease.
    • Hybridoma technology provides a model for studying gene localization in different species.

    Purpose of the Study:

    • To assign the chromosomal locations of specific bovine isozyme loci using mouse myeloma/calf hybridomas.
    • To identify syntenic groups of bovine genes.

    Main Methods:

    • Analysis of bovine isozyme presence in mouse myeloma/calf hybridomas.
    • Correlation with specific banded chromosomes of the bovine complement.

    Main Results:

    • Tentative assignment of the bovine peptidase C (PEP C) isozyme locus to chromosome 5.

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  • Assignment of the bovine lactate dehydrogenase B/peptidase B (LDH B/PEP B) syntenic group to chromosome 19.
  • Evidence suggesting association of LDH A with chromosomes 23-29 and superoxide dismutase 1 (SOD 1) with chromosome 13.

    • Conclusions:

    • Established preliminary chromosomal assignments for several bovine isozymes.
    • Demonstrated the utility of somatic cell hybridization for bovine gene mapping.