Quantification of bands produced by isoelectric focusing using immunoperoxidase

An improved, rapid, and sensitive (to autoradiographic levels) method for the quantification of IgG bands from cerebrospinal fluid is presented. The principle of an internal standard is employed using the discrete bands of a paraprotein to correct for any run to run variability in the enzyme detection system which is linked to the immunoassay of immobilised antigen.