[Chemotherapeutic mixtures for the selective growth of anaerobes]

In order to find a selective medium for the cultivation of Gram-positive spore-less rods several chemotherapeutics have been tested. By adding of 5 micrograms metronidazol/ml to brain-heart-infusion-supplemented agar as well as by putting metronidazol discs on the agar it became possible to cultivate selective test tribes. Lincomycin (5 micrograms/ml), clindamycin (5 micrograms/ml) and novobiocin (5 micrograms/ml) inhibited almost all the test tribes. Spectinomycin (5 micrograms/ml) and neomycin (5 and 10 micrograms/ml) failed to inhibit. With 100 micrograms neomycin/ml Gram-positive anaerobes were clearly inhibited.