[Method of determining low-molecular weight oligoamines in various biological materials]

A modified procedure is described for thin-layer chromatography of dansyl-spermine, -spermidine, -cadaverine and -putrescine. Distinct separation of the dansyl derivatives studied from dansyl-ammonium, which usually causes difficulties, was achieved. This procedure enabled to estimate the pyckomolar quantities of biogenic oligoamines. The method does not require radioactive compounds, enables to carry out the measurements without the use of spectrofluorimeter or fluorescent densitometer. The densitometry of the chromatogram photo-prints was successfully used for quantitative estimation of the oligoamines.