The use of a brucella protein antigen in dermal hypersensitivity as an adjunct method to diagnose bovine brucellosis

A total of 191 cows in 3 herds were injected with a brucella protein antigen in an effort to compare serologic and bacteriologic findings with delayed hypersensitivity and to possibly detect incubative brucellosis. Using cultural and serologic results, the responses to the BPA antigen were inferior to serologic tests in the diagnosis of infection. None of the 23 skin test positive/seronegative cows were positive on subsequent herd blood tests.