Anti-phosphorylcholine antibodies with a preferred reactivity for either PC or PC-phenyl represent independent...

Anti-hapten antibodies to phosphorylcholine(PC)-conjugated proteins can be divided into antibodies reacting preferentially with PC (group I) or with PC-phenyl (group II). We have selected two hybridomas producing group II-type anti-PC antibodies of the IgM and IgE classes, respectively. With one exception, NH2-terminal amino acid sequence analyses of the light chains are identical in their first 21 residues and similar to that of M460, but differ from that of group I-type light chains. These results suggest that PC-phenyl-specific group II antibodies are expressed independently of PC-specific group I antibodies.