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Published on: January 31, 2012

Cellular effects of human leukocyte hydrolases III: inflammatory exudate and synovial fibroblasts

R B Cogen, S B Taubman

    Journal of Oral Pathology
    |June 1, 1984
    PubMed

    Insights

    Human polymorphonuclear leukocyte (PMN) lysosomal enzymes do not destroy synovial fibroblasts. Natural inhibitors in body fluids and inflammatory exudates prevent these enzymes from causing cell damage, suggesting their role in inflammation needs more study.

    Area of Science:

    • Cell Biology
    • Immunology
    • Biochemistry

    Background:

    • Lysosomal enzymes from human polymorphonuclear leukocytes (PMN) are implicated in inflammatory processes.
    • The direct impact of these enzymes on human synovial fibroblasts in inflammatory conditions requires clarification.

    Purpose of the Study:

    • To investigate the potential adverse effects of lysosomal enzymes from human PMNs on the viability of human synovial fibroblasts.
    • To assess the influence of inflammatory exudates on the interaction between PMN enzymes and synovial fibroblasts.

    Main Methods:

    • In vitro experiments exposing human synovial fibroblasts to lysosomal enzymes isolated from human PMNs.
    • Evaluation of enzyme effects in the presence and absence of inflammatory exudate and serum.

    Main Results:

    • Lysosomal enzymes, even at high concentrations, did not cause cell destruction but could detach cells from growth surfaces.
    • This cell detachment effect was inhibited by naturally occurring inhibitors present in serum and inflammatory exudates.
    • Dilute concentrations of inhibitors effectively neutralized the effects of concentrated lysosomal enzymes.

    Conclusions:

    • PMN-derived lysosomal enzymes are unlikely to be direct mediators of synovial fibroblast destruction in inflammatory environments.
    • The presence of natural inhibitors in body fluids and exudates plays a crucial role in mitigating potential damage from these enzymes.
    • Further research is needed to fully elucidate the role of PMN granule enzymes in the cellular pathology of inflammatory diseases.

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