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[Manual method of assaying plasma hemoglobin by tetramethylbenzidine and deferoxamine]
Annales De Biologie Clinique
|January 1, 1984
Summary
This study presents a simple manual assay for measuring low plasma hemoglobin concentrations. The method uses hemoglobin
Area of Science:
- Biochemistry
- Analytical Chemistry
Context:
- Accurate quantification of hemoglobin in biological fluids is crucial for diagnosing various medical conditions.
- Existing methods for measuring low hemoglobin concentrations can be complex or require specialized equipment.
Purpose:
- To develop a simple, rapid, and cost-effective manual method for determining very low concentrations of hemoglobin in plasma.
- To utilize the peroxidase-like activity of hemoglobin for a colorimetric assay.
Summary:
- A manual assay is described for quantifying plasma hemoglobin (0.25–37.3 µmol/l) using hemoglobin's peroxidase activity to decompose hydrogen peroxide.
- The released oxygen oxidizes tetramethylbenzidine, with color stabilization achieved using deferoxamine.
- This technique is quick, reliable, inexpensive, requires no specialized equipment, and is applicable to urine samples.
Impact:
- Provides a practical and accessible method for low-level hemoglobin detection in clinical and research settings.
- Offers a potential alternative for point-of-care testing or resource-limited environments.
- Expands diagnostic capabilities for conditions involving low hemoglobin levels or hemolysis.