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Updated: Aug 17, 2026
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Published on: January 24, 2017
Identification of a 48 S preinitiation complex in reticulocyte lysate
Abstract:
When the polypeptide initiation sequence is interrupted by the peptide antibiotic edeine, the sedimentation coefficient of the native small ribosomal subunit is increased from 43 S to 48 S. Formation of the 48 SN particle is absolutely dependent on the binding of both Met-tRNAf and globin mRNA. These results support the concept that Met-tRNAf binds to the native small ribosomal subunit before mRNA, and that processes involved in mRNA binding to the 43 SN preinitiation complex may be the rate-limiting step of 80 S initiation complex formation.
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