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Related Experiment Videos

Colorimetric assay for acetaminophen in serum.

Z K Shihabi, R M David

    Therapeutic Drug Monitoring
    |January 1, 1984
    PubMed
    Summary

    A new colorimetric method accurately measures acetaminophen in serum using 2-nitroso-1-naphthol-4-sulfonic acid. This sensitive and rapid assay minimizes drug and serum interferences, offering a reliable alternative to HPLC.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Accurate measurement of acetaminophen levels in serum is crucial for therapeutic drug monitoring and overdose management.
    • Existing analytical methods may suffer from interferences or require complex procedures.

    Purpose of the Study:

    • To develop and validate a simple, sensitive, and rapid colorimetric method for acetaminophen determination in human serum.
    • To assess the method's specificity and its correlation with a standard chromatographic technique.

    Main Methods:

    • A colorimetric assay utilizing the reaction between acetaminophen and 2-nitroso-1-naphthol-4-sulfonic acid.
    • Serum samples were analyzed manually and via a semiautomated setup.
    • Comparison with high-performance liquid chromatography (HPLC) for validation.

    Main Results:

    • The developed colorimetric method demonstrated high sensitivity and speed.
    • The assay showed minimal interference from common drugs and the serum matrix.
    • Excellent correlation was observed between the results obtained by the colorimetric method and HPLC.

    Conclusions:

    • This novel colorimetric method provides a reliable, efficient, and interference-free approach for quantifying acetaminophen in serum.
    • The method's simplicity and accuracy make it suitable for both manual and semiautomated clinical laboratory settings.

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