Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Myoglobin determination by high-performance liquid chromatography.

S C Powell, E R Friedlander, Z K Shihabi

    Journal of Chromatography
    |December 28, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Analysis and classification of serum cryoglobulins.

    Methods in molecular medicine·2011
    Same author

    Myoglobin analysis.

    Methods in molecular medicine·2011
    Same author

    Enzyme analysis : cathepsin d as an example.

    Methods in molecular medicine·2011
    Same author

    Serum lamotrigine analysis.

    Methods in molecular medicine·2011
    Same author

    Acetonitrile stacking : serum phenobarbital as an example.

    Methods in molecular medicine·2011
    Same author

    Comparison of glycated albumin and hemoglobin A(1c) levels in diabetic subjects on hemodialysis.

    Kidney international·2008
    Same journal

    Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

    Journal of chromatography·2013
    Same journal

    [Electrophoresis of radionuclides on cellogel].

    Journal of chromatography·2012
    Same journal

    Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

    Journal of chromatography·2010
    Same journal

    Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

    Journal of chromatography·1994
    Same journal

    Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

    Journal of chromatography·1994
    Same journal

    Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

    Journal of chromatography·1994
    See all related articles

    This study presents a simple anion-exchange chromatography method for detecting myoglobin in urine and serum. The method is clinically useful for diagnosing myoglobinuria, identifying elevated myoglobin levels in affected patients.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Clinical Diagnostics

    Background:

    • Myoglobin is a protein found in muscle tissue.
    • Elevated myoglobin levels in urine or serum can indicate muscle damage or myoglobinuria.
    • Accurate and efficient detection methods are crucial for clinical diagnosis.

    Purpose of the Study:

    • To develop and validate an anion-exchange chromatography method for separating and quantifying myoglobin in biological samples.
    • To assess the clinical utility of the method for diagnosing myoglobinuria.

    Main Methods:

    • Anion-exchange chromatography using a slurry-packed column.
    • Direct injection of urine or serum samples.
    • Isocratic elution with Tris buffer.
    • Detection of myoglobin peaks and comparison with horse myoglobin standard.

    Related Experiment Videos

    Main Results:

    • The method successfully separated human muscle myoglobin into two peaks, with one peak increasing upon storage.
    • The latter peak, also observed in horse myoglobin, was typically detected in urine of myoglobinuria patients.
    • Detected urine myoglobin levels in patients ranged from 20 to 3000 mg/l, with undetectable levels in normal subjects.
    • Urine myoglobin was found to be unstable and required immediate analysis.

    Conclusions:

    • The developed anion-exchange chromatography method is a fast, simple, and clinically useful technique for confirming and quantifying myoglobin in patients with myoglobinuria.
    • While not sensitive enough for normal subjects, the method aids in diagnosing myoglobinuria.
    • Further enhancements with more sensitive detectors could broaden the method's applicability.