Application of metabolic profiling to study the effects of ethanol on metabolism in rats

Rat urine was analyzed by both gas chromatography and a combination of gas chromatography/mass spectroscopy in an attempt to apply the technique of metabolic profiling to determine if ethanol consumption produced an alteration in acid excretion products. Rats were fed a liquid diet for seven days then fed ethanol in the same diet. The 24 hr urine for the last day of control and the first day of ethanol differed greatly with respect to four compounds. These were an increase in threonic, glucuronic and an undetermined acid and a decrease in pyroglutamic acid. The biological basis for the alterations was not investigated. Glucuronic acid forms conjugates with many compounds. Possibly an acute dose of ethanol may alter the removal of some compounds from the liver.