In situ localization of S1-labeled actin filaments in chick lens epithelial cells

The actin filaments in the lens epithelial cells of three-day and eight-day post-hatched chicks have been labeled in situ with myosin subfragment 1 (S1). Labeling was accomplished by injuring the lens transcorneally with an ultramicroneedle 5 min or 24 hr before detergent treatment and incubation in S1. A band of filaments found at the epithelio-fiber junction in normal, uninjured chick lens is labeled in the 5 min and 24 hr injury. A subcapsular labeled band is found only in the 24 hr injury, and may be the result of a healing process.